ARG62344

anti-RFP antibody [RF5R]

anti-RFP antibody [RF5R] for Dot blot,ELISA,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
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Overview

Product Description

Mouse Monoclonal antibody [RF5R] recognizes RFP

Tested Reactivity Other
Tested Application Dot, ELISA, ICC/IF, IHC-Fr, IP, WB
Specificity Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc
Host Mouse
Clonality Monoclonal
Clone RF5R
Isotype IgG1
Target Name RFP
Immunogen RFP from the Discosoma sp. (sea anemone) N-terminal peptide-KLH conjugates.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
ICC/IF1:500-1:2000
IHC-FrAssay-dependent
IPAssay-dependent
WB1:1000-1:3000
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note Protein A affinity chromatography from mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7011691 Other gfp

Background Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
DsRed (drFP583) is isolated from Discosoma striata and TurboRFP is isolated from Entacmaea quadricolor (Merzlyak et., al 2007), both proteins are Red fluorescent protein and useful to be reportor genes for cell and animal study.
Highlight Related Antibody Duos and Panels:
ARG30209 Fluorescent-Tags Antibody Panel
Related products:
RFP antibodies; RFP ELISA Kits; RFP Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Customer's Feedback

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Review for anti-RFP antibody [RF5R]

Application:IHC

Sample:Drosophila melanogaster, third instar larvae, wing disc

Sample Preparation Method:Third instar larvae were heat-shock at 37ºC for 1 hr. Dissection in 3 day after heat-shock. Disc were fixed in 4% PFA for 20 min.

Fixation Buffer:4% PFA

Fixation Time:20 min

Fixation Temperature:N/A ºC

Antigen Retrieval Buffer:N/A

Permeabilization Buffer:0.1% Triton-X 100

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC