ARG58777

anti-RFX5 antibody

anti-RFX5 antibody for Western blot and Human,Mouse

Overview

Product Description Goat Polyclonal antibody recognizes RFX5
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog, Pig
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RFX5
Antigen Species Human
Immunogen Synthetic peptide from the internal region of Human RFX5 (NP_000440.1). (C-TGDKSSEPSTLSNE)
Conjugation Un-conjugated
Alternate Names DNA-binding protein RFX5; Regulatory factor X 5

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 73 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53970 Mouse RFX5

GeneID: 5993 Human RFX5

Swiss-port # P48382 Human DNA-binding protein RFX5

Swiss-port # Q9JL61 Mouse DNA-binding protein Rfx5

Gene Symbol RFX5
Gene Full Name regulatory factor X, 5 (influences HLA class II expression)
Background A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]
Function Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. [UniProt]
Calculated MW 65 kDa
PTM Phosphorylated. [UniProt]