ARG63778

anti-RICTOR antibody

anti-RICTOR antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes RICTOR
Tested Reactivity Hu
Predict Reactivity Ms, Dog
Tested Application IHC-P
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RICTOR
Antigen Species Human
Immunogen C-KQPIVDTSAES
Conjugation Un-conjugated
Alternate Names AVO3 homolog; PIA; Rapamycin-insensitive companion of mTOR; hAVO3; AVO3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 253260 Human RICTOR

Swiss-port # Q6R327 Human Rapamycin-insensitive companion of mTOR

Background RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 192 kDa
PTM Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.