ARG42223
anti-RMI2 antibody
anti-RMI2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RMI2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RMI2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human RMI2. (KMTDLSDNPIHESMWELEVEDLHRNIP) |
| Conjugation | Un-conjugated |
| Alternate Names | C16orf75; BLM-associated protein of 18 kDa; BLAP18; RecQ-mediated genome instability protein 2; hRMI2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 19 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3UPE3 Mouse RecQ-mediated genome instability protein 2 Swiss-port # Q96E14 Human RecQ-mediated genome instability protein 2 |
|---|---|
| Gene Symbol | RMI2 |
| Gene Full Name | RecQ mediated genome instability 2 |
| Background | RMI2 is a component of the BLM (RECQL3; MIM 604610) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability (Xu et al., 2008 [PubMed 18923082]).[supplied by OMIM, Nov 2008] |
| Function | Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM. [UniProt] |
| Cellular Localization | Nucleus. Note=Colocalizes with BLM at nuclear DNA repair foci. [UniProt] |
| Calculated MW | 16 kDa |
| PTM | Phosphorylated during mitosis. [UniProt] |
