ARG54635

anti-RNA polymerase II antibody [8WG16]

anti-RNA polymerase II antibody [8WG16] for Western blot and Broad

Gene Regulation antibody; Microbiology and Infectious Disease antibody
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Overview

Product Description

Mouse Monoclonal antibody [8WG16] recognizes RNA polymerase II

Tested Reactivity Broad
Tested Application WB
Specificity This antibody reacts with the highlyconserved heptapeptide repeat of thelargest subunit of eukaryotic RNApolymerase II. It inhibits promoterdirectedtranscription, but it does notinhibit elongation in the nonspecifictranscription assay.
Host Mouse
Clonality Monoclonal
Clone 8WG16
Isotype IgG2a
Target Name RNA polymerase II
Antigen Species Wheat
Immunogen Purified wheat germ RNApolymerase II.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity chromatography
Buffer PBS (pH 7.4)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol rpoB
Gene Full Name RNA polymerase beta chain
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [UniProt]
Research Area Gene Regulation antibody; Microbiology and Infectious Disease antibody
Calculated MW 122 kDa

Customer's Feedback

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Review for anti-RNA polymerase II antibody [8WG16]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Aberrant transcriptional regulations in cancers: genome, transcriptome and epigenome analysis of lung adenocarcinoma cell lines.

ChIP-seq / Human

Suzuki A et al.
Nucleic Acids Res.,  (2014)

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Human cytomegalovirus pUL79 is an elongation factor of RNA polymerase II for viral gene transcription.

Perng YC et al.
PLoS Pathog.,  (2014)

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Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming.

WB / 

Jullien J et al.
Mol Cell.,  (2014)

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Construction of mate pair full-length cDNAs libraries and characterization of transcriptional start sites and termination sites.

ChIP-seq / Human

Matsumoto K et al.
Nucleic Acids Res.,  (2014)

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Differential acetylation of histone H3 at the regulatory region of OsDREB1b promoter facilitates chromatin remodelling and transcription activation during cold stress.

ChIP / Rice

Roy D et al.
PLoS One.,  (2014)

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Rpb4 subunit functions mainly in mRNA synthesis by RNA polymerase II.

WB / 

Schulz D et al.
J Biol Chem.,  (2014)

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Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor.

WB / Arabidopsis thaliana

Wang ZW et al.
Proc Natl Acad Sci U S A.,  (2014)

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A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells.

ChIP-seq / Mouse

Seki M et al.
PLoS One.,  (2014)

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The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.

ChIP / Neurospora crassa

Yang S et al.
Nucleic Acids Res.,  (2014)

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Nonproteolytic roles of 19S ATPases in transcription of CIITApIV genes.

Maganti N et al.
PLoS One.,  (2014)

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Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes.

ChIP / 

Shin MR et al.
PLoS One.,  (2014)

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The TAF9 C-terminal conserved region domain is required for SAGA and TFIID promoter occupancy to promote transcriptional activation.

Saint M et al.
Mol Cell Biol.,  (2014)

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Separation of a functional deubiquitylating module from the SAGA complex by the proteasome regulatory particle.

IP / Saccharomyces cerevisiae

Lim S et al.
Nat Commun.,  (2013)

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Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways.

ChIP / Schizosaccharomyces pombe

Oya E et al.
PLoS Genet.,  (2013)

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Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae.

WB / Saccharomyces cerevisiae

Golla U et al.
PLoS One.,  (2013)

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Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors.

ICC/IF / Arabidopsis thaliana

Kammel C et al.
PLoS One.,  (2013)

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The role of the Arabidopsis Exosome in siRNA-independent silencing of heterochromatic loci.

ChIP / Arabidopsis thaliana

Shin JH et al.
PLoS Genet.,  (2013)

publication_link

 

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CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor.

WB / Mouse

Wu L et al.
Oncogene.,  (2014)

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Mutations in the Arabidopsis H3K4me2/3 demethylase JMJ14 suppress posttranscriptional gene silencing by decreasing transgene transcription.

Le Masson I et al.
Plant Cell.,  (2012)

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Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS.

ChIP / Human

Tao Y et al.
Nucleic Acids Res.,  (2011)

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Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes.

ChIP / Saccharomyces cerevisiae

Knutson BA et al.
Mol Cell Biol.,  (2011)

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The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny.

ChIP / Caenorhabditis elegans

Rechtsteiner A et al.
PLoS Genet.,  (2010)

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Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.

WB / Mouse

Tao Y et al.
PLoS One.,  (2010)

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H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis.

ChIP / Arabidopsis thaliana

Kumar SV et al.
Cell.,  (2010)

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RNA Pol II accumulates at promoters of growth genes during developmental arrest.

WB, ChIP-seq / Caenorhabditis elegans

Baugh LR et al.
Science.,  (2009)

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PolV(PolIVb) function in RNA-directed DNA methylation requires the conserved active site and an additional plant-specific subunit.

WB / Arabidopsis thaliana

Lahmy S et al.
Proc Natl Acad Sci U S A.,  (2009)

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A positive regulatory role for the mSin3A-HDAC complex in pluripotency through Nanog and Sox2.

ChIP / Mouse

Baltus GA et al.
J Biol Chem.,  (2009)

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