ARG41777

anti-RNF20 antibody

anti-RNF20 antibody for Western blot,ICC/IF,Immunoprecipitation,Flow cytometry and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes RNF20
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RNF20
Antigen Species Human
Immunogen Synthetic peptide of Human RNF20.
Conjugation Un-conjugated
Alternate Names EC 6.3.2.-; hBRE1; BRE1A; BRE1-A; RING finger protein 20; BRE1; E3 ubiquitin-protein ligase BRE1A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293
Observed Size ~ 125 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 109331 Mouse RNF20

GeneID: 56254 Human RNF20

Swiss-port # Q5DTM8 Mouse E3 ubiquitin-protein ligase BRE1A

Swiss-port # Q5VTR2 Human E3 ubiquitin-protein ligase BRE1A

Gene Symbol RNF20
Gene Full Name ring finger protein 20, E3 ubiquitin protein ligase
Background The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 114 kDa