ARG41588
anti-RNF220 antibody
anti-RNF220 antibody for Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes RNF220 |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | RNF220 |
Antigen Species | Human |
Immunogen | Recombinant fusion protein corresponding to aa. 267-566 of Human RNF220 (NP_060620.2). |
Conjugation | Un-conjugated |
Alternate Names | RING finger protein 220; E3 ubiquitin-protein ligase RNF220; EC 6.3.2.-; C1orf164 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
Positive Control | Mouse brain | ||||
Observed Size | ~ 72 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q5VTB9 Human E3 ubiquitin-protein ligase RNF220 Swiss-port # Q6PDX6 Mouse E3 ubiquitin-protein ligase Rnf220 |
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Gene Symbol | RNF220 |
Gene Full Name | ring finger protein 220 |
Function | E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. [UniProt] |
Cellular Localization | Cytoplasm. [UniProt] |
Calculated MW | 63 kDa |
PTM | Auto-ubiquitinated; leads to proteasomal degradation. [UniProt] |