ARG63302
anti-RNF34 / RFI antibody
anti-RNF34 / RFI antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes RNF34 / RFI |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RNF34 / RFI |
| Antigen Species | Human |
| Immunogen | KAGATSMWASCC |
| Conjugation | Un-conjugated |
| Alternate Names | CARP-1; E3 ubiquitin-protein ligase RNF34; hRFI; EC 6.3.2.-; Human RING finger homologous to inhibitor of apoptosis protein; RING finger protein RIFF; Caspases-8 and -10-associated RING finger protein 1; FYVE-RING finger protein Momo; RIFF; Caspase regulator CARP1; RIF; RFI; RING finger protein 34; CARP1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 42 kDa |
| PTM | Autoubiquitinated (in vitro). Proteolytically cleaved by caspases upon induction of apoptosis by TNF. |
