ARG66943
anti-ROS1 antibody [SQab22273]
anti-ROS1 antibody [SQab22273] for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab22273] recognizes ROS1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab22273 |
| Isotype | IgG |
| Target Name | ROS1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein fragment of Human ROS1. |
| Conjugation | Un-conjugated |
| Protein Full Name | Proto-oncogene tyrosine-protein kinase ROS |
| Alternate Names | Proto-oncogene c-Ros-1; MCF3; c-ros-1; Receptor tyrosine kinase c-ros oncogene 1; Proto-oncogene tyrosine-protein kinase ROS; c-Ros receptor tyrosine kinase; ROS; EC 2.7.10.1; Proto-oncogene c-Ros |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 52 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human ROS1+ lung carcinoma tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08922 Human Proto-oncogene tyrosine-protein kinase ROS |
|---|---|
| Gene Symbol | ROS1 |
| Gene Full Name | ROS proto-oncogene 1 , receptor tyrosine kinase |
| Background | This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008] |
| Function | Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Research Area | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 264 kDa |
| PTM | Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11. |
