ARG42970

anti-RP2 / XRP2 antibody [3D7]

anti-RP2 / XRP2 antibody [3D7] for Western blot,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [3D7] recognizes RP2 / XRP2
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 3D7
Isotype IgG2b
Target Name RP2 / XRP2
Antigen Species Human
Immunogen Recombinant protein corresponding to D244-M348 of Human RP2 / XRP2.
Conjugation Un-conjugated
Alternate Names TBCCD2; Protein XRP2; XRP2; DELXp11.3; NM23-H10; NME10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6102 Human RP2

Swiss-port # O75695 Human Protein XRP2

Gene Symbol RP2
Gene Full Name retinitis pigmentosa 2 (X-linked recessive)
Background The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly-folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death [provided by RefSeq, Jul 2008]
Function Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, cilium. Note=Detected predominantly at the plasma membrane of rod and cone photoreceptors. Not detected in the nucleus. [UniProt]
Calculated MW 40 kDa
PTM Myristoylated on Gly-2; which may be required for membrane targeting.

Palmitoylated on Cys-3; which may be required for plasma membrane targeting (Probable). Mutation of Cys-3 targets the protein to internal membranes. [UniProt]