ARG59083
anti-RRM2 antibody
anti-RRM2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RRM2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody might cross-react to RRM2B protein based on sequence analysis. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RRM2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-389 of Human RRM2 (NP_001025.1). |
| Conjugation | Un-conjugated |
| Alternate Names | R2; EC 1.17.4.1; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RR2M; RR2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Mouse thymus | ||||||||||
| Observed Size | 45kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RRM2 |
| Gene Full Name | ribonucleotide reductase M2 |
| Background | This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009] |
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. [UniProt] |
