ARG43327

anti-RSPO1 / R spondin 1 antibody

anti-RSPO1 / R spondin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RSPO1 / R spondin 1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RSPO1 / R spondin 1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 21-263 of Human RSPO1 / R spondin 1 (NP_001033722.1).
Conjugation Un-conjugated
Alternate Names hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 192199 Mouse RSPO1

GeneID: 284654 Human RSPO1

Swiss-port # Q2MKA7 Human R-spondin-1

Swiss-port # Q9Z132 Mouse R-spondin-1

Gene Symbol RSPO1
Gene Full Name R-spondin 1
Background This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Function Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805). [UniProt]
Cellular Localization Secreted. Nucleus. Note=Seems to mainly localize to nucleoli. [UniProt]
Calculated MW 29 kDa