ARG42159

anti-RTCB / HSPC117 antibody

anti-RTCB / HSPC117 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes RTCB / HSPC117
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Gpig, Hrs, Rb, Zfsh
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RTCB / HSPC117
Antigen Species Human
Immunogen Synthetic peptide around the N-terminal region of Human RTCB / HSPC117. (within the following region: PEAVV SPGGV GFDIN CGVRL LRTNL DESDV QPVKE QLAQA MFDHI PVGVG)
Conjugation Un-conjugated
Alternate Names EC 6.5.1.3; HSPC117; DJ149A16.6; C22orf28; tRNA-splicing ligase RtcB homolog; FAAP

Application Instructions

Predict Reactivity Note Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
Application Suggestion
Tested Application Dilution
IHC-P10 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size ~ 55 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS, 0.09% (w/v) Sodium azide and 2% Sucrose.
Preservative 0.09% (w/v) Sodium azide
Stabilizer 2% Sucrose
Concentration Batch dependent: 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51493 Human RTCB

Swiss-port # Q9Y3I0 Human tRNA-splicing ligase RtcB homolog

Gene Symbol RTCB
Gene Full Name RNA 2',3'-cyclic phosphate and 5'-OH ligase
Function Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. [UniProt]
Cellular Localization Nucleus. Cytoplasm. Note=Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low. [UniProt]
Calculated MW 55 kDa