ARG41062

anti-RUVBL1 / Pontin antibody

anti-RUVBL1 / Pontin antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RUVBL1 / Pontin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RUVBL1 / Pontin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-214 of Human RUVBL1 (NP_003698.1).
Conjugation Un-conjugated
Alternate Names ECP-54; Pontin 52; 49 kDa TATA box-binding protein-interacting protein; ECP54; TIP49A; NMP238; EC 3.6.4.12; RuvB-like 1; TIH1; TIP49a; TIP60-associated protein 54-alpha; NMP 238; PONTIN; INO80 complex subunit H; Nuclear matrix protein 238; RVB1; INO80H; 54 kDa erythrocyte cytosolic protein; 49 kDa TBP-interacting protein; Pontin52; TIP49; TAP54-alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse spleen
Observed Size 55 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56505 Mouse RUVBL1

GeneID: 65137 Rat RUVBL1

GeneID: 8607 Human RUVBL1

Gene Symbol RUVBL1
Gene Full Name RuvB-like AAA ATPase 1
Function Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation.

May be able to bind plasminogen at cell surface and enhance plasminogen activation. [UniProt]
Cellular Localization Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. [UniProt]
Calculated MW 50 kDa