ARG41899

anti-Rab5 antibody

anti-Rab5 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Monkey

Overview

Product Description Goat Polyclonal antibody recognizes Rab5
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Rab5
Antigen Species Mouse
Immunogen Purified recombinant peptides within aa. 115 to the C-terminus of Mouse Rab5a, Rab5b and Rab5c.
Conjugation Un-conjugated
Alternate Names RAB5A: Ras-related protein Rab-5A; RAB5
RAB5B: Ras-related protein Rab-5B
RAB5C: RAB5L; L1880; RAB5CL; RABL; Ras-related protein Rab-5C

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:250
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:250 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Spleen
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol RAB5A; RAB5B; RAB5C
Gene Full Name RAB5A, member RAS oncogene family
RAB5B, member RAS oncogene family
RAB5C, member RAS oncogene family
Background Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]). [supplied by OMIM, Nov 2010]
Function RAB5A: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. [UniProt]

RAB5B and RAB5C: Protein transport. Probably involved in vesicular traffic. [UniProt]
Cellular Localization RAB5A: Cytosol, Endosome and Plasma membrane. [UniProt]
RAB5B: Endosome and Plasma membrane. [UniProt]
RAB5C: Endosome and Plasma membrane. [UniProt]
Calculated MW 24 kDa