ARG41899
anti-Rab5 antibody
anti-Rab5 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Monkey
Overview
Product Description | Goat Polyclonal antibody recognizes Rab5 |
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Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Rab5 |
Antigen Species | Mouse |
Immunogen | Purified recombinant peptides within aa. 115 to the C-terminus of Mouse Rab5a, Rab5b and Rab5c. |
Conjugation | Un-conjugated |
Alternate Names | RAB5A: Ras-related protein Rab-5A; RAB5 RAB5B: Ras-related protein Rab-5B RAB5C: RAB5L; L1880; RAB5CL; RABL; Ras-related protein Rab-5C |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Positive Control | Spleen | ||||||||||
Observed Size | ~ 26 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
Preservative | 0.05% Sodium azide |
Stabilizer | 20% Glycerol |
Concentration | 3 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Gene Symbol | RAB5A; RAB5B; RAB5C |
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Gene Full Name | RAB5A, member RAS oncogene family RAB5B, member RAS oncogene family RAB5C, member RAS oncogene family |
Background | Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]). [supplied by OMIM, Nov 2010] |
Function | RAB5A: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. [UniProt] RAB5B and RAB5C: Protein transport. Probably involved in vesicular traffic. [UniProt] |
Cellular Localization | RAB5A: Cytosol, Endosome and Plasma membrane. [UniProt] RAB5B: Endosome and Plasma membrane. [UniProt] RAB5C: Endosome and Plasma membrane. [UniProt] |
Calculated MW | 24 kDa |