ARG56558

anti-Rad51 antibody

anti-Rad51 antibody for Western blot and Human,Mouse,Rat,Monkey,Hamster

Overview

Product Description Mouse Monoclonal antibody recognizes Rad51
Tested Reactivity Hu, Ms, Rat, Hm, Mk
Tested Application WB
Specificity This antibody detects endogenous levels of RAD51 and does not cross-react with related proteins.
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Target Name Rad51
Antigen Species Human
Immunogen Recombinant protein of Human Rad51.
Conjugation Un-conjugated
Alternate Names DNA repair protein RAD51 homolog 1; HsT16930; FANCR; BRCC5; RECA; HRAD51; hRAD51; HsRAD51; MRMV2; RAD51 homolog A; RAD51A; HsRad51

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.03% Proclin-300 and 50% Glycerol.
Preservative 0.03% Proclin-300
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19361 Mouse RAD51

GeneID: 5888 Human RAD51

Swiss-port # Q06609 Human DNA repair protein RAD51 homolog 1

Swiss-port # Q08297 Mouse DNA repair protein RAD51 homolog 1

Gene Symbol RAD51
Gene Full Name RAD51 recombinase
Background The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Function Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. [UniProt]
Calculated MW 37 kDa
PTM Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.