ARG63242

anti-Rad51C antibody

anti-Rad51C antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes Rad51C
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognise all reported human isoforms.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Rad51C
Antigen Species Human
Immunogen RGKTFRFEMQRDL-C
Conjugation Un-conjugated
Alternate Names DNA repair protein RAD51 homolog 3; RAD51 homolog C; RAD51L2; RAD51-like protein 2; BROVCA3; R51H3; FANCO

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5889 Human RAD51C

Swiss-port # O43502 Human DNA repair protein RAD51 homolog 3

Background This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 42 kDa