ARG63733
anti-Retinoic Acid Receptor alpha antibody
anti-Retinoic Acid Receptor alpha antibody for Western blot and Human
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Retinoic Acid Receptor alpha |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Specificity | NP_000955.1 (isfa) and NP_001138773.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Retinoic Acid Receptor alpha |
| Antigen Species | Human |
| Immunogen | C-SPSLSPSSHRSSPATQSP |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear receptor subfamily 1 group B member 1; RAR; RAR-alpha; Retinoic acid receptor alpha; NR1B1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010] |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 51 kDa |
| PTM | Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. Ubiquitinated. |
