ARG59919

anti-RhoF antibody

anti-RhoF antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes RhoF
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RhoF
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 40-120 of Human RhoF (NP_061907.2).
Conjugation Un-conjugated
Alternate Names RIF; Rho-related GTP-binding protein RhoF; ARHF; Rho in filopodia; Rho family GTPase Rif

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size 27 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23912 Mouse RHOF

GeneID: 54509 Human RHOF

Swiss-port # Q8BYP3 Mouse Rho-related GTP-binding protein RhoF

Swiss-port # Q9HBH0 Human Rho-related GTP-binding protein RhoF

Gene Symbol RHOF
Gene Full Name ras homolog family member F (in filopodia)
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. [UniProt]
Calculated MW 24 kDa

Images (1) Click the Picture to Zoom In

  • ARG59919 anti-RhoF antibody WB image

    Western blot: 25 µg of 293T cell lysate stained with ARG59919 anti-RhoF antibody at 1:1000 dilution.