ARG52417

anti-Rhodopsin antibody [1D4]

anti-Rhodopsin antibody [1D4] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunocytochemistry,Immunohistochemistry (PFA perfusion fixed frozen sections),Immunoprecipitation,Western blot and Amphibians,Cow,Human,Mammal,Mouse,Rat,Zebrafish

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [1D4] recognizes Rhodopsin
Tested Reactivity Hu, Ms, Rat, Amph, Cow, Mamm, Zfsh
Predict Reactivity Rb
Tested Application ELISA, ICC, IHC-FoFr , IHC-Fr, IHC-P, IP, WB
Specificity The antibody reacts to C- terminal epitope TETSQVAPA- (COOH) of rhodopsin, so it also reacts to C9 tag (TETSQVAPA).
Host Mouse
Clonality Monoclonal
Clone 1D4
Isotype IgG1
Target Name Rhodopsin
Antigen Species Bovine
Immunogen Purified native bovine rhodopsin
Epitope Antibody binds to the C- terminal epitope-T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin.
Conjugation Un-conjugated
Alternate Names Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC1:1000
IHC-FoFr 1:1000
IHC-FrAssay-dependent
IHC-P1:100 - 1:1000
IPAssay-dependent
WB1:1000
Application Note Specific for the ~ 39k rhodopsin protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 212541 Mouse RHO

GeneID: 24717 Rat RHO

GeneID: 30295 Zebrafish RHO

Gene Symbol RHO
Gene Full Name rhodopsin
Background Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 39 kDa
PTM Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.

Clone References

Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium.

Wavre-Shapton ST et al.
J Cell Sci.,  (2014)

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Effective delivery of large genes to the retina by dual AAV vectors.

IHC-Fr / Mouse

Trapani I et al.
EMBO Mol Med.,  (2014)

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Myosin7a deficiency results in reduced retinal activity which is improved by gene therapy.

EM / Mouse

Colella P et al.
PLoS One.,  (2013)

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CERKL knockdown causes retinal degeneration in zebrafish.

IHC-Fr / Zebrafish

Riera M et al.
PLoS One.,  (2013)

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Reproducible and sustained regulation of Gαs signalling using a metazoan opsin as an optogenetic tool.

ICC/IF / Human

Bailes HJ et al.
PLoS One.,  (2012)

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Degeneration of cortical function in the Royal College of Surgeons rat.

IHC-FoFr / Rat

Gias C et al.
Vision Res.,  (2011)

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A neural-specific F-box protein Fbs1 functions as a chaperone suppressing glycoprotein aggregation.

IP / Human

Yoshida Y et al.
J Biol Chem.,  (2007)

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Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.

IP / Bovine

Hodges RS et al.
J Biol Chem.,  (1988)

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