ARG22271

anti-Rhodopsin antibody [4D2]

anti-Rhodopsin antibody [4D2] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,ELISA and Amphibians,Bird,Fish,Mammal,Shark,Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [4D2] recognizes Rhodopsin
Tested Reactivity Hu, Ms, Rat, Amph, Bird, Fsh, Mamm, Shark
Species Does Not React With Invt
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Host Mouse
Clonality Monoclonal
Clone 4D2
Isotype IgG1
Target Name Rhodopsin
Antigen Species Bovine
Immunogen Bovine Rhodopsin
Conjugation Un-conjugated
Alternate Names Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC1:1000
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 212541 Mouse RHO

GeneID: 24717 Rat RHO

GeneID: 6010 Human RHO

Gene Symbol RHO
Gene Full Name rhodopsin
Background Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. [UniProt]
Cellular Localization Membrane
Calculated MW 39 kDa
PTM Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.