ARG41451

anti-Rhodopsin antibody

anti-Rhodopsin antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Rhodopsin
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Rhodopsin
Antigen Species Human
Immunogen Synthetic peptide of Human Rhodopsin.
Conjugation Un-conjugated
Alternate Names Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat eyeball
Observed Size 37 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24717 Rat RHO

GeneID: 6010 Human RHO

Swiss-port # P08100 Human Rhodopsin

Swiss-port # P51489 Rat Rhodopsin

Gene Symbol RHO
Gene Full Name rhodopsin
Background Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. [UniProt]
Cellular Localization Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia. [UniProt]
Calculated MW 39 kDa
PTM Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Contains one covalently linked retinal chromophore. [UniProt]