ARG11145

anti-Rhodopsin antibody [B630]

anti-Rhodopsin antibody [B630] for Western blot,ICC/IF,IHC-Frozen sections and Human,Rat,Mouse,Cow,Pig,Horse

Overview

Product Description Mouse Monoclonal antibody [B630] recognizes Rhodopsin
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone B630
Isotype IgG1
Target Name Rhodopsin
Antigen Species Bovine
Immunogen Purified bovine rhodopsin.
Conjugation Un-conjugated
Alternate Names Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 33 kDa

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 212541 Mouse RHO

GeneID: 24717 Rat RHO

GeneID: 397437 Pig RHO

Gene Symbol RHO
Gene Full Name rhodopsin
Background The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]
Function Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343). [UniProt]
Cellular Localization Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia. [UniProt]
Calculated MW 39 kDa
PTM Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Contains one covalently linked retinal chromophore. [UniProt]