ARG11145
anti-Rhodopsin antibody [B630]
anti-Rhodopsin antibody [B630] for Western blot,ICC/IF,IHC-Frozen sections and Human,Rat,Mouse,Cow,Pig,Horse
Overview
| Product Description | Mouse Monoclonal antibody [B630] recognizes Rhodopsin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B630 |
| Isotype | IgG1 |
| Target Name | Rhodopsin |
| Antigen Species | Bovine |
| Immunogen | Purified bovine rhodopsin. |
| Conjugation | Un-conjugated |
| Alternate Names | Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 33 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RHO |
| Gene Full Name | rhodopsin |
| Background | The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017] |
| Function | Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343). [UniProt] |
| Cellular Localization | Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia. [UniProt] |
| Calculated MW | 39 kDa |
| PTM | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore. [UniProt] |
