ARG62619

anti-Rhodopsin antibody [RET-P1]

anti-Rhodopsin antibody [RET-P1] for ICC/IF,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Immunoprecipitation and Mouse,Rat,Bovine,Human

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [RET-P1] recognizes Rhodopsin
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone RET-P1
Isotype IgG1
Target Name Rhodopsin
Antigen Species Rat
Immunogen Membrane preparation from adult rat retina.
Conjugation Un-conjugated
Alternate Names Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2

Application Instructions

Application Note FACS: 1µg for 106 cells
WB: 1 - 2 µg/ml
IHC-P: 1/2 - 1/4
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IMR-5 cells, brain or retina tissue sections.

Properties

Form Liquid
Purification Protein G purified
Buffer 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 212541 Mouse RHO

GeneID: 24717 Rat RHO

GeneID: 509933 Bovine RHO

Gene Symbol Rho
Gene Full Name rhodopsin
Background Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
Function Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity). [UniProt]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 39 kDa
PTM Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.

Clone References

Transplantation of human bone marrow mesenchymal stem cells as a thin subretinal layer ameliorates retinal degeneration in a rat model of retinal dystrophy.

IHC-P / 

Tzameret A et al.
Exp Eye Res.,  (2014)

publication_link

 

hr_line

Conditional ablation of the choroideremia gene causes age-related changes in mouse retinal pigment epithelium.

ICC/IF / Mouse

Wavre-Shapton ST et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.

WB / Mouse

Chucair-Elliott AJ et al.
J Biol Chem.,  (2012)

publication_link

 

hr_line

Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina.

IHC-P / Mouse

Vekslin S et al.
Mol Vis.,  (2010)

publication_link

 

hr_line

Immunocytochemical study of rhodopsin-containing putative encephalic photoreceptors in house sparrow, Passer domesticus.

IHC / House Sparrow

Wang G et al.
Gen Comp Endocrinol.,  (2011)

publication_link

 

hr_line

IMP dehydrogenase type 1 associates with polyribosomes translating rhodopsin mRNA.

IP / Cow

Mortimer SE et al.
J Biol Chem.,  (2008)

publication_link

 

hr_line

Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation.

IHC / Dog

Tuntivanich N et al.
Invest Ophthalmol Vis Sci.,  (2009)

publication_link

 

hr_line

Conversion of mammalian Müller glial cells into a neuronal lineage by in vitro aggregate-culture.

IHC / Rat

Kubota A et al.
Biochem Biophys Res Commun.,  (2006)

publication_link

 

hr_line