ARG51437

anti-Ring1A antibody

anti-Ring1A antibody for Western blot and Human,Mouse,Rat

Gene Regulation antibody; Polycomb Complexes antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Ring1A
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ring1A
Antigen Species Human
Immunogen Peptide sequence around aa.35~39( I-A-V-S-P) derived from Human Ring1A.
Conjugation Un-conjugated
Alternate Names RING finger protein 1; Polycomb complex protein RING1; EC 6.3.2.-; Really interesting new gene 1 protein; RING1A; RNF1; E3 ubiquitin-protein ligase RING1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19763 Mouse RING1

GeneID: 309626 Rat RING1

GeneID: 6015 Human RING1

Gene Symbol RING1
Gene Full Name ring finger protein 1
Background Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Function Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30309 Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2)
Related products:
Ring1A antibodies; Ring1A Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Gene Regulation antibody; Polycomb Complexes antibody
Calculated MW 42 kDa