ARG64072
anti-Robo1 / DUTT1 antibody
anti-Robo1 / DUTT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Cancer antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Robo1 / DUTT1 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Robo1 / DUTT1 |
| Antigen Species | Mouse |
| Immunogen | C-QKARPAKKQKHQ |
| Conjugation | Un-conjugated |
| Alternate Names | Deleted in U twenty twenty; H-Robo-1; DUTT1; Roundabout homolog 1; SAX3 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Robo1 |
| Gene Full Name | roundabout homolog 1 (Drosophila) |
| Background | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). May be required for lung development. [UniProt] |
| Research Area | Cancer antibody; Neuroscience antibody |
| Calculated MW | 181 kDa |
| PTM | Ubiquitinated. May be deubiquitinated by USP33. |
