ARG54640
anti-S100 antibody [B32.1]
anti-S100 antibody [B32.1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Bovine,Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [B32.1] recognizes S100 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | ICC/IF, IHC-P |
| Specificity | This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B32.1 |
| Isotype | IgG2a |
| Target Name | S100 |
| Antigen Species | Bovine |
| Immunogen | bovine brain S100 protein |
| Conjugation | Un-conjugated |
| Alternate Names | S-100 protein beta chain; NEF; S100; Protein S100-B; S100 calcium-binding protein B; S100beta; S-100 protein subunit beta; S100-B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Melanoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | S100B |
| Gene Full Name | S100 calcium binding protein B |
| Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008] |
| Function | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 11 kDa |
Clone References
Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?
IHC /
Insulin-like growth factor I messenger RNA and protein are expressed in the human lymph node and distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and endothelial cells.
IHC-P / Human
Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice.
IHC-FoFr / Mouse
Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development.
ICC/IF / Human
An extract (THC-002) of Ba-Wei-Die-Huang-Wan inhibits expression of tachykinins, and P2X3 and TRPV1 receptors, and inhibits ATP-induced detrusor overactivity in spontaneously hypertensive rats.
IHC-FoFr / Rat
