ARG56521

anti-S1P1 / EDG1  antibody

anti-S1P1 / EDG1  antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes S1P1 / EDG1 
Tested Reactivity Hu, Ms, Rat, Pig
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name S1P1 / EDG1 
Antigen Species Human
Immunogen Synthetic peptide around  aa. 241-253 of Human S1P1 / EDG-1. (ISKASRSSEKSLA)
Conjugation Un-conjugated
Alternate Names D1S3362; CD antigen CD363; EDG1; CD363; EDG-1; S1P1; Endothelial differentiation G-protein coupled receptor 1; S1P receptor Edg-1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1-phosphate receptor 1; CHEDG1; ECGF1; S1P receptor 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IHC-P1:200
WB1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13609 Mouse S1PR1

GeneID: 1901 Human S1PR1

GeneID: 29733 Rat S1PR1

Gene Symbol S1PR1
Gene Full Name sphingosine-1-phosphate receptor 1
Background The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]
Function G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury. [UniProt]
Calculated MW 43 kDa
PTM S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.