ARG51849

anti-S6 Ribosomal Protein phospho (Ser235 / 236) antibody

anti-S6 Ribosomal Protein phospho (Ser235 / 236) antibody for Western blot and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein phospho (Ser235 / 236)
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name S6 Ribosomal Protein
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 235/236 (R-L-S(p)-S(p)-L-R) derived from Human S6 Ribosomal Protein.
Conjugation Un-conjugated
Alternate Names Phosphoprotein NP33; 40S ribosomal protein S6; S6

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20104 Mouse RPS6

GeneID: 29304 Rat RPS6

GeneID: 6194 Human RPS6

Gene Symbol RPS6
Gene Full Name ribosomal protein S6
Background May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Function May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 29 kDa
PTM Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.