ARG66614

anti-SAE1 / AOS1 antibody

anti-SAE1 / AOS1 antibody for Western blot,ICC/IF and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes SAE1 / AOS1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SAE1 / AOS1
Antigen Species Human
Immunogen Synthetic peptide within aa. 190-270 of Human SAE1 / AOS1.
Conjugation Un-conjugated
Alternate Names UBLE1A; SUMO-activating enzyme subunit 1; AOS1; HSPC140; SUA1; Ubiquitin-like 1-activating enzyme E1A

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10055 Human SAE1

Swiss-port # Q9UBE0 Human SUMO-activating enzyme subunit 1

Gene Symbol SAE1
Gene Full Name SUMO1 activating enzyme subunit 1
Background Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 38 kDa