ARG51807
anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody
anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody
Publication6
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Specificity | The antibody detects endogenous levels of dually phosphorylated JNK1 / JNK2 at Thr183 and Tyr185, and JNK3 at Thr221 and Tyr223. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SAPK / JNK |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V) derived from Human SAPK / JNK. |
| Conjugation | Un-conjugated |
| Alternate Names | JNK3A; Mitogen-activated protein kinase 10; p54bSAPK; Stress-activated protein kinase 1b; JNK3; c-Jun N-terminal kinase 3; Stress-activated protein kinase JNK3; EC 2.7.11.24; SAPK1b; p493F12; MAP kinase 10; PRKM10; MAPK 10; MAP kinase p49 3F12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MAPK10 |
| Gene Full Name | mitogen-activated protein kinase 10 |
| Background | Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30205 NFkB Activation Antibody Panel ARG30294 Phospho SAPK / JNK Antibody Duo (Total, pT183/Y185) Related products: JNK antibodies; JNK ELISA Kits; JNK Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Treatment of Obesity with Celastrol Tumor microenvironments are shown to affect progression of several cancer subtypes Immune signaling protein TLR4 has opposing roles in breast cancer development Understanding Your Cells: Choose the right markers Related poster download: Toll-like Receptor.pdf |
| Research Area | Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody |
| Calculated MW | 53 kDa |
| PTM | Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development. |
Customer's Feedback
Excellent
Review for anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody
Application：WB
Sample：HeLa cells untreated or treated with TNF-alpha
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody
Application：WB
Sample：HeLa treated with 5 ng/ml TNF-a for 1) 5 min, 2) 10 min, and 3) 20 min.
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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A Natural Dietary Flavone Myricetin as an α-Hemolysin Inhibitor for Controlling Staphylococcus aureus Infection.
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The fucoidan from sea cucumber Apostichopus japonicus attenuates lipopolysaccharide-challenged liver injury in C57BL/6J mice.
WB / Mouse
The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells
WB / Human
Mice deficient in aldo-keto reductase 1a (Akr1a) are resistant to thioacetamide-induced liver injury.
WB / Mouse
