ARG59081

anti-SDHA antibody

anti-SDHA antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SDHA
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SDHA
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 385-664 of Human SDHA (NP_004159.2).
Conjugation Un-conjugated
Alternate Names FP; Fp; SDH1; PGL5; SDH2; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; CMD1GG; EC 1.3.5.1; SDHF

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U-251MG
Observed Size 70kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 157074 Rat SDHA

GeneID: 6389 Human SDHA

GeneID: 66945 Mouse SDHA

Gene Symbol SDHA
Gene Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Background This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Function Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
Calculated MW 73 kDa
PTM Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.

Acetylated. Deacetylated by SIRT3. [UniProt]