ARG65002

anti-SDHAF1 antibody

anti-SDHAF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Metabolism antibody

Overview

Product Description Goat Polyclonal antibody recognizes SDHAF1
Tested Reactivity Hu
Predict Reactivity Ms, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SDHAF1
Antigen Species Human
Immunogen C-HDSTGAPETRPDGR
Conjugation Un-conjugated
Alternate Names Succinate dehydrogenase assembly factor 1, mitochondrial; SDHAF1; SDH assembly factor 1; LYRM8; LYR motif-containing protein 8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 644096 Human SDHAF1

Swiss-port # A6NFY7 Human Succinate dehydrogenase assembly factor 1, mitochondrial

Background The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010]
Highlight Related products:
Anti-Goat IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Metabolism antibody
Calculated MW 13 kDa