ARG63827

anti-SDHB antibody

anti-SDHB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes SDHB
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SDHB
Antigen Species Human
Immunogen C-ATYKEKKASV
Conjugation Un-conjugated
Alternate Names Ip; SDH2; SDH1; IP; EC 1.3.5.1; PGL4; CWS2; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; SDHIP; Iron-sulfur subunit of complex II; SDH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6390 Human SDHB

Swiss-port # P21912 Human Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Background Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 32 kDa