ARG66824
anti-SDHB antibody [SQab20236]
anti-SDHB antibody [SQab20236] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20236] recognizes SDHB |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20236 |
| Isotype | IgG |
| Target Name | SDHB |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human SDHB. |
| Conjugation | Un-conjugated |
| Alternate Names | Ip; SDH2; SDH1; IP; EC 1.3.5.1; PGL4; CWS2; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; SDHIP; Iron-sulfur subunit of complex II; SDH |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21912 Human Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial |
|---|---|
| Gene Symbol | SDHB |
| Gene Full Name | succinate dehydrogenase complex, subunit B, iron sulfur (Ip) |
| Background | Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008] |
| Function | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt] |
| Calculated MW | 32 kDa |
