ARG66824

anti-SDHB antibody [SQab20236]

anti-SDHB antibody [SQab20236] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab20236] recognizes SDHB
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab20236
Isotype IgG
Target Name SDHB
Antigen Species Human
Immunogen Synthetic peptide derived from Human SDHB.
Conjugation Un-conjugated
Alternate Names Ip; SDH2; SDH1; IP; EC 1.3.5.1; PGL4; CWS2; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; SDHIP; Iron-sulfur subunit of complex II; SDH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6390 Human SDHB

Swiss-port # P21912 Human Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene Symbol SDHB
Gene Full Name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Background Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]
Function Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
Calculated MW 32 kDa