ARG23274

anti-SERPINB9 antibody [7D8]

anti-SERPINB9 antibody [7D8] for IHC-Frozen sections,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [7D8] recognizes SERPINB9
Mouse anti Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 has been reported to work in western blotting applications. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirst et al. 2003).
Tested Reactivity Hu
Species Does Not React With Ms, Pig
Tested Application FACS, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 7D8
Isotype IgG1
Target Name SERPINB9
Antigen Species Human
Immunogen Recombinant SERPINB9 produced in P. pastoris.
Conjugation Un-conjugated
Alternate Names Cytoplasmic antiproteinase 3; Peptidase inhibitor 9; CAP-3; PI-9; PI9; Serpin B9; CAP3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:100
IHC-FrAssay-dependent
IHC-P1:50
WBAssay-dependent
Application Note FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5272 Human SERPINB9

Swiss-port # P50453 Human Serpin B9

Gene Symbol SERPINB9
Gene Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 9
Background This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]
Function Granzyme B inhibitor. [UniProt]
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Calculated MW 42 kDa