ARG63166

anti-SET / I2 alpha PP2A antibody

anti-SET / I2 alpha PP2A antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes SET / I2 alpha PP2A
Tested Reactivity Hu
Predict Reactivity Cow
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize isoform 2 (NP_003002.2).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SET / I2 alpha PP2A
Antigen Species Human
Immunogen SAPAAKVSKKELNS-C
Conjugation Un-conjugated
Alternate Names I-2PP2A; Template-activating factor I; I2PP2A; Inhibitor of granzyme A-activated DNase; Protein SET; TAF-IBETA; IPP2A2; TAF-I; PHAPII; 2PP2A; Phosphatase 2A inhibitor I2PP2A; IGAAD; HLA-DR-associated protein II

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6418 Human SET

Swiss-port # Q01105 Human Protein SET

Background The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 33 kDa
PTM Isoform 2 is phosphorylated on Ser-15 and Ser-24.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity).
Isoform 2 is cleaved after Lys-176 by GZMA. The cleavage inhibits its nucelosome assembly activity and disrupts the inhibition on NME1.