ARG58984

anti-SETD7 antibody

anti-SETD7 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SETD7
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SETD7
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-366 of Human SETD7 (NP_085151.1).
Conjugation Un-conjugated
Alternate Names SET7/9; KMT7; Lysine N-methyltransferase 7; SET domain-containing protein 7; SET7; EC 2.1.1.43; Histone-lysine N-methyltransferase SETD7; Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; SET9

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse pancreas
Observed Size 41 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 73251 Mouse SETD7

GeneID: 80854 Human SETD7

Swiss-port # Q8VHL1 Mouse Histone-lysine N-methyltransferase SETD7

Swiss-port # Q8WTS6 Human Histone-lysine N-methyltransferase SETD7

Gene Symbol SETD7
Gene Full Name SET domain containing (lysine methyltransferase) 7
Function Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProt]
Cellular Localization Chromosome, Nucleus. [UniProt]
Calculated MW 41 kDa