ARG40015
anti-SF3B6 antibody
anti-SF3B6 antibody for Western blot and Human,Mouse
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SF3B6 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SF3B6 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-125 of Human SF3B6 (NP_057131.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Pre-mRNA branch site protein p14; Splicing factor 3B subunit 6; CGI-110; Spliceosome-associated protein, 14-kDa; SF3B14; SF3B14a; HSPC175; SF3b 14 kDa subunit; P14; Ht006; Splicing factor 3b, subunit 6, 14kDa; SAP14; SAP14a |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse spleen and K562 | ||||
| Observed Size | 14 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SF3B6 |
| Gene Full Name | splicing factor 3b, subunit 6, 14kDa |
| Background | This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008] |
| Function | Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 15 kDa |
Specific References
The long non-coding RNA DKFZp434J0226 regulates the alternative splicing process through phosphorylation of SF3B6 in PDAC
WB / Human
