ARG42131
anti-SH3GL1 / EEN antibody
anti-SH3GL1 / EEN antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes SH3GL1 / EEN |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SH3GL1 / EEN |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human SH3GL1 / EEN. (C-EPFDLGEPEQSN) (NP_003016.1) |
| Conjugation | Un-conjugated |
| Alternate Names | SH3P8; Extra eleven-nineteen leukemia fusion gene protein; Endophilin-A2; CNSA1; EEN; SH3 domain protein 2B; Endophilin-2; EEN fusion partner of MLL; SH3D2B; SH3 domain-containing GRB2-like protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | K562 | ||||||
| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SH3GL1 |
| Gene Full Name | SH3-domain GRB2-like 1 |
| Background | This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| Function | Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Early endosome membrane; Peripheral membrane protein. Cell projection, podosome. Note=Associated with postsynaptic endosomes in hippocampal neurons. [UniProt] |
| Calculated MW | 41 kDa |
