ARG56196
anti-SHBG antibody [SHBG/245]
anti-SHBG antibody [SHBG/245] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [SHBG/245] recognizes SHBG |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SHBG/245 |
| Isotype | IgG1 |
| Target Name | SHBG |
| Antigen Species | Human |
| Immunogen | Recombinant full-length human SHBG protein. |
| Conjugation | Un-conjugated |
| Alternate Names | SHBG; SBP; Testosterone-estradiol-binding globulin; Sex hormone-binding globulin; Sex steroid-binding protein; ABP; TeBG; Testis-specific androgen-binding protein; Testosterone-estrogen-binding globulin; TEBG |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris buffer with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SHBG |
| Gene Full Name | sex hormone-binding globulin |
| Background | This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. [UniProt] |
| Cellular Localization | Cytoplasmic & extracellular (secreted) |
| Calculated MW | 44 kDa |
| PTM | Variant Asn-356 contains one N-linked (GlcNAc...) at position 356. |
