ARG63185
anti-SIAH1 antibody
anti-SIAH1 antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SIAH1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Specificity | This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SIAH1 |
| Antigen Species | Human |
| Immunogen | SRQTATALPTGTSKC |
| Conjugation | Un-conjugated |
| Alternate Names | Seven in absentia homolog 1; Siah-1; EC 6.3.2.-; SIAH1A; Siah-1a; E3 ubiquitin-protein ligase SIAH1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| Calculated MW | 31 kDa |
| PTM | Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2. |
