ARG63644

anti-SIAHBP1 / FIR antibody

anti-SIAHBP1 / FIR antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes SIAHBP1 / FIR
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1)
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SIAHBP1 / FIR
Antigen Species Human
Immunogen C-YDQERFDNSDLSA
Conjugation Un-conjugated
Alternate Names FIR; RoBPI; Poly; SIAHBP1; Siah-binding protein 1; U; 60 kDa poly; FUSE-binding protein-interacting repressor; VRJS; Siah-BP1; Ro-binding protein 1; RoBP1; FBP-interacting repressor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22827 Human PUF60

Swiss-port # Q9UHX1 Human Poly(U)-binding-splicing factor PUF60

Background The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 60 kDa