ARG40164

anti-SIN3A antibody

anti-SIN3A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SIN3A
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SIN3A
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human SIN3A.
Conjugation Un-conjugated
Alternate Names Transcriptional corepressor Sin3a; Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size 150 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20466 Mouse SIN3A

GeneID: 25942 Human SIN3A

Swiss-port # Q60520 Mouse Paired amphipathic helix protein Sin3a

Swiss-port # Q96ST3 Human Paired amphipathic helix protein Sin3a

Gene Symbol SIN3A
Gene Full Name SIN3 transcription regulator family member A
Background The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]
Function Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in he control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. [UniProt]
Cellular Localization Nucleus. Nucleus, nucleolus. Note=Recruited to the nucleolus by SAP30L. [UniProt]
Calculated MW 145 kDa
PTM SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2. [UniProt]