ARG56580

anti-SIRT6 antibody

Ordering Information

Package

Price (USD)

100 μl

355.00

50 μl

245.00

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Datasheet
Review (4)

Overview

Product Description	Mouse Monoclonal antibody recognizes SIRT6
Tested Reactivity	Hu, Ms, Rat, Mk
Tested Application	ICC/IF, IP, WB
Specificity	This antibody detects endogenous levels of SIRT6 and does not cross-react with related proteins.
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Target Name	SIRT6
Antigen Species	Human
Immunogen	Purified recombinant Human SIRT6 protein expressed in E. coli.
Conjugation	Un-conjugated
Alternate Names	SIR2L6; NAD-dependent protein deacetylase sirtuin-6; SIR2-like protein 6; Regulatory protein SIR2 homolog 6; EC 3.5.1.-

Application Instructions

Application Suggestion

Tested Application	Dilution
ICC/IF	1:100
IP	Assay-dependent
WB	1:500

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Observed Size

~ 42 kDa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.4), 0.03% Proclin-300 and 50% Glycerol.
Preservative	0.03% Proclin-300
Stabilizer	50% Glycerol
Concentration	2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 50721 Mouse SIRT6 GeneID: 51548 Human SIRT6 Swiss-port # P59941 Mouse NAD-dependent protein deacetylase sirtuin-6 Swiss-port # Q8N6T7 Human NAD-dependent protein deacetylase sirtuin-6
Gene Symbol	SIRT6
Gene Full Name	sirtuin 6
Background	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]
Function	NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. [UniProt]
Calculated MW	39 kDa