ARG59227

anti-SKAP55 antibody

anti-SKAP55 antibody for IHC-Formalin-fixed paraffin-embedded sections and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SKAP55
Tested Reactivity Rat
Predict Reactivity Hu, Hm
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SKAP55
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 163-189 of Human SKAP55. (RMAPHLRRDSKKESCFELTSQDRRSYE)
Conjugation Un-conjugated
Alternate Names Src family-associated phosphoprotein 1; SCAP1; Src kinase-associated phosphoprotein 1; SKAP55; pp55; Src kinase-associated phosphoprotein of 55 kDa; HEL-S-81p; SKAP-55

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 286975 Rat SKAP1

Swiss-port # Q4V7G1 Rat Src kinase-associated phosphoprotein 1

Gene Symbol SKAP1
Gene Full Name src kinase associated phosphoprotein 1
Background This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]
Function Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Cell membrane. Note=Upon T-cell stimulation, translocates to lipid rafts at the cell membrane. [UniProt]
Calculated MW 41 kDa
PTM Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC (Probable). [UniProt]