ARG59227
anti-SKAP55 antibody
anti-SKAP55 antibody for IHC-Formalin-fixed paraffin-embedded sections and Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes SKAP55 |
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Tested Reactivity | Rat |
Predict Reactivity | Hu, Hm |
Tested Application | IHC-P |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | SKAP55 |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa. 163-189 of Human SKAP55. (RMAPHLRRDSKKESCFELTSQDRRSYE) |
Conjugation | Un-conjugated |
Alternate Names | Src family-associated phosphoprotein 1; SCAP1; Src kinase-associated phosphoprotein 1; SKAP55; pp55; Src kinase-associated phosphoprotein of 55 kDa; HEL-S-81p; SKAP-55 |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
Preservative | 0.05% Sodium azide |
Stabilizer | 5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q4V7G1 Rat Src kinase-associated phosphoprotein 1 |
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Gene Symbol | SKAP1 |
Gene Full Name | src kinase associated phosphoprotein 1 |
Background | This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008] |
Function | Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. [UniProt] |
Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=Upon T-cell stimulation, translocates to lipid rafts at the cell membrane. [UniProt] |
Calculated MW | 41 kDa |
PTM | Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC (Probable). [UniProt] |