ARG45136
anti-SLC12A1 / NKCC2 antibody
anti-SLC12A1 / NKCC2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SLC12A1 / NKCC2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Target Name | SLC12A1 / NKCC2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to N-terminal region of human SLC12A1 / NKCC2. |
| Conjugation | Un-conjugated |
| Alternate Names | BSC1; potassium; Solute carrier family 12 member 1; Kidney-specific Na-K-Cl symporter; Bumetanide-sensitive sodium-; NKCC2; CCC2; BSC; Bumetanide-Sensitive Cotransporter; Na-K-2Cl Cotransporter |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 150-290 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SLC12A1 |
| Gene Full Name | Solute carrier family 12 member 1 |
| Background | This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010] |
| Function | Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. [UniProt] |
| Cellular Localization | Membrane; Multi-pass membrane protein; Apical cell membrane. [UniProt] |
| Calculated MW | 121 kDa |
| PTM | Glycoprotein; Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45136 anti-SLC12A1 / NKCC2 antibody IHC-P image
Immunohistochemistry: Human renal cancer tissue stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 2 μg/ml.
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ARG45136 anti-SLC12A1 / NKCC2 antibody ICC/IF image
Immunofluorescence: Rat kidney tissue. stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 5 μg/ml.
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ARG45136 anti-SLC12A1 / NKCC2 antibody WB image
Western blot: Monkey kidney tissue stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 0.5 μg/ml.
