ARG22199
anti-SLC12A3 / NCC antibody
anti-SLC12A3 / NCC antibody for Electron microscopy,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SLC12A3 / NCC |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog |
| Tested Application | EM, ICC/IF, IHC-P, WB |
| Specificity | Detects ~160kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | SLC12A3 / NCC |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around aa. 74-95 (N-terminus) of Rat NCC |
| Conjugation | Un-conjugated |
| Alternate Names | Solute carrier family 12 member 3; TSC; Na-Cl cotransporter; Thiazide-sensitive sodium-chloride cotransporter; Na-Cl symporter; NCCT; NCC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Slc12a3 |
| Gene Full Name | solute carrier family 12 (sodium/chloride transporter), member 3 |
| Background | This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. [UniProt] |
| Cellular Localization | Membrane |
| Calculated MW | 113 kDa |
| PTM | Ubiquitinated; ubiquitination is essential for regulation of endocytosis. The BCR(KLHL3) complex was initially identified as a candidate ubiquitin ligase for SLC12A3 (PubMed:22406640). However, it was later shown that it is not the case. Phosphorylated in response to IL18. |
Specific References
High-salt diet accelerates bone loss accompanied by activation of ion channels related to kidney and bone tissue in ovariectomized rats
WB / Mouse
