ARG57892

anti-SM22 alpha / Transgelin antibody

anti-SM22 alpha / Transgelin antibody for Western blot,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes SM22 alpha / Transgelin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SM22 alpha / Transgelin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-201 of Human Transgelin (NP_001001522.1).
Conjugation Un-conjugated
Alternate Names SM22-alpha; 22 kDa actin-binding protein; Smooth muscle protein 22-alpha; Protein WS3-10; Transgelin; WS3-10; TAGLN1; SMCC; SM22

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart
Observed Size 20 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21345 Mouse TAGLN

GeneID: 25123 Rat TAGLN

GeneID: 6876 Human TAGLN

Gene Symbol TAGLN
Gene Full Name transgelin
Background The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody
Calculated MW 23 kDa