ARG40290
anti-SM22 alpha / Transgelin antibody [TAGLN/247]
anti-SM22 alpha / Transgelin antibody [TAGLN/247] for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [TAGLN/247] recognizes SM22 alpha / Transgelin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TAGLN/247 |
| Isotype | IgG1, kappa |
| Target Name | SM22 alpha / Transgelin |
| Antigen Species | Human |
| Immunogen | Recombinant full-length Human SM22 alpha / Transgelin. |
| Conjugation | Un-conjugated |
| Alternate Names | SM22-alpha; 22 kDa actin-binding protein; Smooth muscle protein 22-alpha; Protein WS3-10; Transgelin; WS3-10; TAGLN1; SMCC; SM22 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0), for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TAGLN |
| Gene Full Name | transgelin |
| Background | The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody |
| Calculated MW | 23 kDa |
